How to Choose Fresh Fish Using Physical, Chemical, and Microbiological Indicators

By. Lutfi - 28 Aug 2025

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How to Choose Fresh Fish Using Physical, Chemical, and Microbiological Indicators

Kelolalaut.com Fish is one of the most nutritious sources of protein, vitamins, and essential fatty acids. However, not all fish available in the market is of the same quality. Choosing fresh fish is crucial not only for taste and texture but also for safety, since spoiled fish can harbor harmful bacteria or toxic substances. To make an informed decision, three major parameters should be considered: physical indicators, chemical indicators, and microbiological indicators. By understanding these aspects, consumers, chefs, and even food industry professionals can ensure that the fish they select is both safe and of high quality.

Physical Indicators: What the Eye Can See

The physical condition of a fish is the most immediate way to judge its freshness. Some common parameters to observe include

1. Appearance of the eyes: Fresh fish should have clear, bright, and bulging eyes. Cloudy, sunken, or dull eyes are signs that the fish is no longer fresh.

  • Convex eyeballs, clear cornea and pupil, shiny and typical of the species (Score 9)
  • Flat eyeballs, slightly cloudy cornea, grayish pupil, moderately shiny (Score 7)
  • Sunken eyeballs, cloudy cornea, grayish pupil, dull and non-shiny (Score 5)

2. Skin and scales: A fresh fish will have shiny, metallic skin with firmly attached scales. Dry skin or loose scales indicate aging or mishandling.

  • Transparent, bright, and shiny mucus layer (Score 9)
  • Slightly cloudy mucus layer (Score 7)
  • Thick mucus layer (especially in marine fish), discolored (Score 5)

3. Flesh texture: When pressed lightly, the flesh of a fresh fish should spring back quickly. Soft, mushy, or slimy flesh shows decomposition.

  • Very bright flesh cuts, species-specific, very firm tissue (Score 9)
  • Moderately bright flesh cuts, firm tissue (Score 7)
  • Faded flesh cuts, weak tissue structure (Score 5)
  • Dense and compact (Score 9)
  • Dense but less compact (Score 7)
  • Loose and non-compact (Score 5

4. Gills: Bright red or pink gills are a reliable freshness indicator. Brownish or grey gills with foul odor suggest spoilage.

  • Bright blood-red or reddish-brown with slightly cloudy mucus (Score 9)
  • Dark red or reddish-brown, bright with very little transparent mucus (Score 7)
  • Gray or grayish-brown with turbid mucus (Score 5)

5. Odor: Fresh fish smells like seawater or has a mild, clean scent. A strong “fishy” or ammonia-like smell indicates deterioration

  • Very fresh, strong species-specific odor (Score 9)
  • Fresh, mild species-specific odor (Score 7)
  • Neutral, slightly sour odor (Score 5)

The higher the average organoleptic score, the better the fish quality. Conversely, lower scores indicate poorer quality. These physical indicators are the easiest for consumers to apply directly when shopping in markets or supermarkets.

Chemical Indicators: The Science Behind Freshness

Beyond appearance, chemical parameters provide a deeper insight into the fish’s quality. These indicators are often used in laboratories and processing plants but can also inform consumer awareness:

  • pH level: Fresh fish usually has a slightly acidic to neutral pH. As spoilage progresses, the pH rises due to microbial activity and the breakdown of proteins.
  • Total Volatile Basic Nitrogen (TVB-N): This parameter measures compounds such as ammonia and trimethylamine, which are released as the fish decomposes. Higher TVB-N values mean reduced freshness.
  • Biogenic amines (like histamine): Improperly stored fish, especially species like mackerel and tuna, may accumulate histamine. This can cause food poisoning if consumed.
  • Fat oxidation: Fish rich in omega-3 fatty acids are prone to oxidation, leading to rancid flavors. Antioxidant protection and proper storage are important to preserve chemical quality.

While consumers cannot directly measure these parameters at home, awareness of proper storage, transportation, and labeling can help in making safer choices.

Microbiological Indicators: The Hidden Factor

Fish is highly perishable due to microbial activity. Microbiological parameters are crucial to assess the safety of fish:

  • Total Plate Count (TPC): This measures the number of bacteria present in the fish. A high bacterial count indicates spoilage and possible safety risks.
  • Pathogenic bacteria: Common hazards include Salmonella, Vibrio, Listeria monocytogenes, and Escherichia coli. These can cause severe foodborne illnesses if consumed.
  • Spoilage bacteria: Species such as Pseudomonas and Shewanella are responsible for unpleasant odors and texture changes.

Microbiological tests are usually carried out by food industries or health authorities, but consumers can still reduce risks by buying from trusted sources, checking expiry dates, and ensuring proper storage at home.

Practical Tips for Consumers

  • Buy fish from reputable sellers who maintain proper hygiene and cold storage.
  • Look for clear labels, including harvest or packaging dates, especially for frozen fish.
  • Store fish at 0–4°C and consume it within a short period. For longer storage, freezing is recommended.
  • Cook fish thoroughly to eliminate harmful microorganisms.

Choosing fresh fish is both an art and a science. While physical indicators are easily observed by consumers, chemical and microbiological parameters reveal the deeper truth about fish quality and safety. By combining all three aspects—physical, chemical, and microbiological indicators—we can ensure that the fish we bring to our table is not only delicious but also safe and nutritious. With this knowledge, consumers can shop more confidently and reduce the risk of foodborne illnesses, while also enjoying the maximum flavor and health benefits that fish has to offer.

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